Electron-nuclear double resonance spectroscopy (and electron spin-echo envelope modulation spectroscopy) in bioinorganic chemistry

Electron-nuclear double resonance spectroscopy (and electron spin-echo envelope modulation spectroscopy) in bioinorganic chemistry.

This perspective discusses the ways that advanced paramagnetic resonance techniques, namely electron-nuclear double resonance (ENDOR) and electron spin-echo envelope modulation (ESEEM) spectroscopies, can help us understand how metal ions function in biological systems.

Nuclear modulation effects in ‘‘211’’ electron spin-echo correlation spectroscopy

This work represents the synthesis of the 211 pulse sequence for the study of electron spin-echo envelope modulation ~ESEEM! with the technique of spin-echo correlated spectroscopy ~SECSY!, which has previously been used to study nuclear modulation by two-dimensional Fourier transform ESR methods. This example of ‘‘pulse adjustable’’ spectroscopy, wherein the pulse width and pulse amplitude of ...

Advanced paramagnetic resonance spectroscopies of iron-sulfur proteins: Electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM).

The advanced electron paramagnetic resonance (EPR) techniques, electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) spectroscopies, provide unique insights into the structure, coordination chemistry, and biochemical mechanism of nature's widely distributed iron-sulfur cluster (FeS) proteins. This review describes the ENDOR and ESEEM techniques and then pr...

Supporting Information Cross-Polarization Electron-Nuclear Double Resonance Spectroscopy

5- and 6-pulse electron spin echo envelope modulation (ESEEM) of multi-nuclear spin systems.

In 3-pulse ESEEM and the original 4-pulse HYSCORE, nuclei with large modulation depth (k approximately 1) suppress spectral peaks from nuclei with weak modulations (k approximately 0). This cross suppression can impede the detection of the latter nuclei, which are often the ones of interest. We show that two extended pulse sequences, 5-pulse ESEEM and 6-pulse HYSCORE, can be used as experimenta...